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Arabidopsis protocols / edited by Julio Salinas and Jose J. Sanchez-Serrano.

Contributor(s): Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.) ; v. 323.Publication details: Totowa, N.J. : Humana Press, c2006.Edition: 2nd edDescription: xv, 469 p., 6 p. of plates : ill. (some col.) ; 27 cmISBN:
  • 1588293955 (alk. paper)
  • 1597450030 (eISBN)
  • 9781588293954
Subject(s): LOC classification:
  • QK495.C9 A735 2006
NLM classification:
  • SB 608.A7
Contents:
Growth of plants and preservation of seeds / Luz Rivero-Lepinckas, Deborah Crist, and Randy Scholl -- Prevention and control of pests and diseases / Jenifer Bush, Georg Jander, and Frederick M. Ausubel -- Growth of other species related to Arabidopsis thaliana / Krithika Yogeeswaran and Mikhail Elia Nasrallah -- Grafting / Katherine Bainbridge ... [et al.] -- Synchronization, transformation, and cryopreservation of suspension-cultured cells / Margit Menges and James A.H. Murray -- Genetic analysis / Maarten Koornneeff, Carlos Alonso-Blanco, and Piet Stam -- QTL analysis / Carlos Alonso-Blanco, Maarten Koornneeff, and Johan W. van Ooijen --
EMS mutagenesis of arabidopsis / YoungSig Kim, Karen S. Schumaker, and Jian-Kang Zhu -- Low-resolution mapping of untagged mutations / M. Rosa Ponce ... [et al.] -- Gene identification and cloning by molecular marker mapping / Georg Jander -- High-throughput TILLING for arabidopsis / Bradley J. Till ... [et al.] -- Genotyping and mapping with high-density oligonucleotide arrays / Justin Borevitz -- Forward genetic screening of insertional mutants / Anja Schneider and Dario Leister -- PCR-based screening for insertional mutants / Anna N. Stepanova and Jose M. Alonso -- Cytogenetic analysis of arabidopsis / Martin Lysak, Paul Fransz, and Ingo Schubert -- Using information from public arabidopsis databases to aid research / Margarita Garcia-Hernandez and Leonore Reiser -- In planta agrobacterium-mediated transformation by vacuum infiltration / Brian W. Tague and Joanna Mantis -- Transient expression assay by agroinfiltration of leaves / Min Woo Lee and Yinong Yang -- Functional analysis of transcription factors by microparticle bombardment / Ida Ruberti, Giovanna Sessa, and Giorgio Morelli -- Transfection assays with protoplasts containing integrated reporter genes / Shiv Tiwari ... [et al.] -- Chloroplast genetic engineering via organogenesis or somatic embryogenesis / Amit Dhingra and Henry Daniell -- [Beta]-Glucuronidase as reporter gene: advantages and limitations / Kye-Won Kim ... [et al.] -- Use of fluorescent proteins as reporters / Guido Jach -- Luciferases as reporter genes / Megan M. Southern, Paul E. Brown, and Anthony Hall -- Aqueorin as reporter gene / Christoph Plieth --
Applications of chemical-inducible expression systems in functional genomics and biotechnology / Jianru Zuo, Peter D. Hare, and Nam-Hai Chua -- RNA extraction / Huazhong Shi and Ray Bressan -- Target preparation for DNA microarray hybridization / Tong Zhu, Sherman H. Chang, and Pedro Gil -- Statistical issues in microarray data analysis / Willem A. Rensink and Samuel P. Hazen -- Preparation and quality assesment of RNA from cell-specific samples obtained by laser microdissection / Regine Kleber and Julia Kehr -- Protein isolation and second-dimension electrophoretic separation / Bernhard Schlesier and Hans-Peter Mock -- Isolation of nuclear proteins / Tomasz T. Calikowski and Iris Meier -- Puification and fractionation of membranes for proteomic analysis / Anne Marmagne ... [et al.] -- Protein microsequencing / Alberto Paradela and Juan Pablo Albar -- Peptide-based phosphoproteomics with immobilized metal ion chromatography / Thomas S. Nühse and Scott C. Peck -- Metabolite profiling in arabidopsis / Oliver Fiehn -- Hormone profiling in arabidopsis / Axel Müller, Pedra Düchting, and Elmar W. Weiler.
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Includes bibliographical references and index.

Growth of plants and preservation of seeds / Luz Rivero-Lepinckas, Deborah Crist, and Randy Scholl -- Prevention and control of pests and diseases / Jenifer Bush, Georg Jander, and Frederick M. Ausubel -- Growth of other species related to Arabidopsis thaliana / Krithika Yogeeswaran and Mikhail Elia Nasrallah -- Grafting / Katherine Bainbridge ... [et al.] -- Synchronization, transformation, and cryopreservation of suspension-cultured cells / Margit Menges and James A.H. Murray -- Genetic analysis / Maarten Koornneeff, Carlos Alonso-Blanco, and Piet Stam -- QTL analysis / Carlos Alonso-Blanco, Maarten Koornneeff, and Johan W. van Ooijen --

EMS mutagenesis of arabidopsis / YoungSig Kim, Karen S. Schumaker, and Jian-Kang Zhu -- Low-resolution mapping of untagged mutations / M. Rosa Ponce ... [et al.] -- Gene identification and cloning by molecular marker mapping / Georg Jander -- High-throughput TILLING for arabidopsis / Bradley J. Till ... [et al.] -- Genotyping and mapping with high-density oligonucleotide arrays / Justin Borevitz -- Forward genetic screening of insertional mutants / Anja Schneider and Dario Leister -- PCR-based screening for insertional mutants / Anna N. Stepanova and Jose M. Alonso -- Cytogenetic analysis of arabidopsis / Martin Lysak, Paul Fransz, and Ingo Schubert -- Using information from public arabidopsis databases to aid research / Margarita Garcia-Hernandez and Leonore Reiser -- In planta agrobacterium-mediated transformation by vacuum infiltration / Brian W. Tague and Joanna Mantis -- Transient expression assay by agroinfiltration of leaves / Min Woo Lee and Yinong Yang -- Functional analysis of transcription factors by microparticle bombardment / Ida Ruberti, Giovanna Sessa, and Giorgio Morelli -- Transfection assays with protoplasts containing integrated reporter genes / Shiv Tiwari ... [et al.] -- Chloroplast genetic engineering via organogenesis or somatic embryogenesis / Amit Dhingra and Henry Daniell -- [Beta]-Glucuronidase as reporter gene: advantages and limitations / Kye-Won Kim ... [et al.] -- Use of fluorescent proteins as reporters / Guido Jach -- Luciferases as reporter genes / Megan M. Southern, Paul E. Brown, and Anthony Hall -- Aqueorin as reporter gene / Christoph Plieth --

Applications of chemical-inducible expression systems in functional genomics and biotechnology / Jianru Zuo, Peter D. Hare, and Nam-Hai Chua -- RNA extraction / Huazhong Shi and Ray Bressan -- Target preparation for DNA microarray hybridization / Tong Zhu, Sherman H. Chang, and Pedro Gil -- Statistical issues in microarray data analysis / Willem A. Rensink and Samuel P. Hazen -- Preparation and quality assesment of RNA from cell-specific samples obtained by laser microdissection / Regine Kleber and Julia Kehr -- Protein isolation and second-dimension electrophoretic separation / Bernhard Schlesier and Hans-Peter Mock -- Isolation of nuclear proteins / Tomasz T. Calikowski and Iris Meier -- Puification and fractionation of membranes for proteomic analysis / Anne Marmagne ... [et al.] -- Protein microsequencing / Alberto Paradela and Juan Pablo Albar -- Peptide-based phosphoproteomics with immobilized metal ion chromatography / Thomas S. Nühse and Scott C. Peck -- Metabolite profiling in arabidopsis / Oliver Fiehn -- Hormone profiling in arabidopsis / Axel Müller, Pedra Düchting, and Elmar W. Weiler.